Invention Grant
- Patent Title: Method for knock-in of DNA into target region of mammalian genome, and cell
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Application No.: US15528506Application Date: 2015-11-17
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Publication No.: US10362771B2Publication Date: 2019-07-30
- Inventor: Tomoji Mashimo , Kazuto Yoshimi , Takehito Kaneko
- Applicant: KYOTO UNIVERSITY
- Applicant Address: JP Kyoto
- Assignee: KYOTO UNIVERSITY
- Current Assignee: KYOTO UNIVERSITY
- Current Assignee Address: JP Kyoto
- Agency: Leydig, Voit & Mayer, Ltd.
- Priority: JP2014-235898 20141120
- International Application: PCT/JP2015/082279 WO 20151117
- International Announcement: WO2016/080399 WO 20160526
- Main IPC: C12N15/63
- IPC: C12N15/63 ; C12N15/00 ; C07H21/04 ; C07K14/00 ; A01K67/027 ; C12N5/10 ; C12N15/09 ; C12N15/90 ; C07K14/315

Abstract:
This invention provides a method for knock-in of a donor DNA into the genome of a cell, comprising introducing at least one artificial nuclease system capable of cleaving target sequence(s) of the cell genome, the donor DNA, and two single-stranded oligonucleotides (ssODNs) into the cell,the artificial nuclease system cleaving the target sequence(s) on the cell genome, the two ssODNs each complementary to one of the ends generated by the target sequence cleavage in the cell genome and to one of the introduction ends of the donor DNA, the donor DNA being knocked-in at the cleavage site via the two ssODNs.
Public/Granted literature
- US20170251647A1 METHOD FOR KNOCK-IN OF DNA INTO TARGET REGION OF MAMMALIAN GENOME, AND CELL Public/Granted day:2017-09-07
Information query
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