Exemplary embodiments are directed to biometric enrollment systems including a camera and an image analysis module. The camera configured is to capture a probe image of a subject, the probe image including an iris of the subject. The image analysis module is configured to determine an iris characteristic of the iris in the probe image. The image analysis module is configured to analyze the probe image relative to a first enrollment image to determine if a match exists based on the iris characteristic. If the match exists, the image analysis module is configured to electronically store the matched probe image as an accepted image. The image analysis module is configured to select and establish the accepted image as a second enrollment image if the accepted image meets enrollment image criteria.