- Patent Title: Detection method for mutation in 93rd amino acid of hepatitis C virus NS5A protein, and detection kit for mutation in 93rd amino acid of hepatitis C virus NS54 protein
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Application No.: US15103184Application Date: 2014-12-11
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Publication No.: US10370730B2Publication Date: 2019-08-06
- Inventor: Satoshi Mochida , Yoshihito Uchida , Jun-ichi Kouyama , Kayoko Naiki
- Applicant: SAITAMA MEDICAL UNIVERSITY
- Applicant Address: JP Iruma-Gun, Saitama
- Assignee: SAITAMA MEDICAL UNIVERSITY
- Current Assignee: SAITAMA MEDICAL UNIVERSITY
- Current Assignee Address: JP Iruma-Gun, Saitama
- Agency: Buchanan, Ingersoll & Rooney PC
- Priority: JP2013255748 20131211; JP2014185917 20140912
- International Application: PCT/JP2014/082773 WO 20141211
- International Announcement: WO2015/087945 WO 20150618
- Main IPC: C12Q1/70
- IPC: C12Q1/70

Abstract:
A method for detecting a mutation in an amino acid at position 93 of a hepatitis C virus NS5A protein, the method including: synthesizing cDNA using, as a template, hepatitis C virus RNA in a sample; and performing a real-time PCR with a cycling probe method using, as a template, the cDNA; wherein a primer set used in the real-time PCR is a certain primer set; and wherein probes used in the real-time PCR include certain probes.
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