An electronic device having a buffering positioning mechanism includes a casing, an internal module and at least one buffer member. The casing includes two shells. Internal components of the electronic device are all provided on a carrier and are assembled to form the internal module. The internal module is accommodated in the casing, and is fixed to the casing without using any fixing elements. The buffer member fills and supports between an inner wall of the casing and an outer wall of the internal module, wherein the inner wall and the outer wall are opposite each other. Thus, collision resistance and fall resistance as well as an effect of fixing the internal module in the casing without using any fixing elements are achieved.