Invention Grant
- Patent Title: Purifying insulin using cation exchange and reverse phase chromatography in the presence of an organic modifier and elevated temperature
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Application No.: US15124080Application Date: 2015-03-11
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Publication No.: US10407483B2Publication Date: 2019-09-10
- Inventor: Allison D. Ortigosa , Michael P. Coleman , Shibu T. George , Michael A. Rauscher , Mark C. Sleevi , Kartoa Chow
- Applicant: Merck Sharp & Dohme Corp.
- Applicant Address: US NJ Rahway
- Assignee: Merck Sharp & Dohme Corp.
- Current Assignee: Merck Sharp & Dohme Corp.
- Current Assignee Address: US NJ Rahway
- Agent John David Reilly; Laura M. Ginkel
- International Application: PCT/US2015/019864 WO 20150311
- International Announcement: WO2015/138548 WO 20150917
- Main IPC: C07K14/62
- IPC: C07K14/62 ; C07K1/18 ; C07K1/20
Abstract:
A process is described for purifying insulin and insulin analogs that comprises high-pressure liquid chromatography with an acidic cation exchange medium performed in the presence of a water miscible organic modifier and at an elevated temperature followed by reverse phase chromatography performed in the presence of a water miscible organic modifier and at an elevated temperature.
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