A wrong-way vehicle prevention system utilizes a sensor to detect a wrong-way vehicle traveling in a wrong direction on an off-ramp, and activates a disabling portion having a tire-rupture apparatus that ruptures one or more tires of the wrong-way vehicle. An alert portion having a sign, alert lights and/or an audible alert feature may be activated when a wrong-way vehicle is detected and the wrong-way vehicle may stop and turn around before driving over the tire rupture apparatus. The tire rupture apparatus has a rupture plate that extend substantially across the off-ramp and pivots up to raise a rupture portion above the surface of the off-ramp. The rupture portion has a sharp edge that will rupture a tire. The wrong-way vehicle system may alert authorities when a wrong-way vehicle is detected. The wrong-way vehicle prevention system may utilize an integral power supply to enable locating the system in remote areas.