[Object] To provide an information processing apparatus that is capable of presenting navigation information for ophthalmic surgery at high speed and with high precision. [Solving Means] An information processing apparatus according to the present technology includes a magnification acquisition unit and an image generation unit. The magnification acquisition unit acquires a magnification of a target image with respect to a reference image, the reference image being an image of an eye to be treated, the target image being an image of the eye picked up at a time different from an imaging time of the reference image. The image generation unit generates a navigation image including navigation information for guiding treatment of the eye on the basis of the magnification acquired by the magnification acquisition unit.