A microfluidic analysis device and manufacturing method are provided. The device includes a capillary substrate, a cover substrate adjacent to a cover side of the capillary substrate and/or a bottom substrate adjacent to a bottom side of the capillary substrate, a capillary structure with at least one capillary in the interior of the capillary substrate and/or at the interface of the capillary substrate with the cover substrate and/or with the bottom substrate, and a fluid-conducting arrangement for conducting a fluid through the capillary structure. The arrangement may be designed for compartmenting the fluid using controlled pressure pulses. A linear sensor element extends toward and/or away from and/or along the capillary, a fluid contact end of which and at least an adjacent part of its feed lie in the plane of the capillary, and may be integrated in the device.