A touch substrate and its manufacturing method thereof, a display device, a fingerprint determination device and a method for determining a fingerprint are provided. The touch substrate touch driving electrodes, touch sensing electrodes and signal lines on a base substrate which at least includes a first region and a second region. The plurality of touch driving electrodes include a plurality of first touch driving electrodes in the first region and a plurality of second touch driving electrodes in the second region, and each first touch driving electrode and one second touch driving electrode are connected with a same signal line; and/or the plurality of touch sensing electrodes include a plurality of first touch sensing electrodes in the first region and a plurality of second touch sensing electrodes in the second region, and each first touch sensing electrode and one second touch sensing electrode are connected with a same signal line.