An interlock data collection and calibration system has a device computer, and a gas sample delivery system for delivering a first gas sample and a second gas sample to the ignition interlock device, the first and second gas samples having different predetermined concentrations of alcohol. The device computer includes a calibration program for calibrating the ignition interlock device using the first gas sample, and then delivering the second gas sample to the ignition interlock device to verify that the ignition interlock device correctly determines the second alcohol concentration of the second sample gas.