A driving method of a visual interface system is disclosed. The visual interface system includes an operation apparatus and a matrix display apparatus having a display surface and a matrix substrate. The matrix substrate has a substrate and a matrix disposed at one side of the substrate while the display surface is located at another side of the substrate. The driving method includes steps of: transmitting a plurality of encoded signals and a plurality of display signals by the matrix substrate of the matrix display apparatus; and receiving at least one of the encoded signals by the operation apparatus operating on the display surface. This approach allows the visual interface system to be equipped with display and communication functions without configuring an additional touch input panel, so that the products can be lighter and thinner and have lower manufacturing cost.