Embodiments of the present disclosure provide a method and a device for cancelling an echo, and a computer readable storage medium. The device includes a loudspeaker configured to play an acoustic signal corresponding to an analog audio signal. The device further includes a microphone configured to convert a mixed acoustic signal received into a mixed audio signal. The mixed acoustic signal includes an echo of the acoustic signal played and an acoustic signal from a user. The device further includes an analog-to-digital converter configured to convert the analog audio signal into a digital signal as an echo reference signal. The device further includes an echo canceller, configured to cancel an echo component from the mixed audio signal using the echo reference signal to obtain a user audio signal corresponding to the acoustic signal from the user.