The present application discloses an organic light-emitting display panel and a manufacturing method thereof, and an organic light-emitting display device. The organic light-emitting display panel comprises a substrate, a first electrode layer, a second electrode layer, an organic light-emitting functional layer formed between the first electrode layer and the second electrode layer and comprising a plurality of first optical adjustment units, a plurality of second optical adjustment units and at least one light emitting layer covering a display area of the organic light-emitting display panel, and a pixel definition layer partitioning the organic light-emitting functional layer to form a pixel array comprising a first color pixel, a second color pixel and a third color pixel in an array arrangement.