A lighting control system and a method thereof are provided. The lighting control system includes a light board and a mainboard. The light board includes a light-emitting module, transmission interfaces and a lighting control module. The mainboard outputs interface testing signals to inquire which types of the transmission interfaces are supported by the light board. The transmission interfaces output interface message signals in response to the interface testing signal. When the mainboard determines that the light board supports one or more types of the transmission interfaces indicated by the interface testing signal, the mainboard selects one of the transmission interfaces and instructs the selected transmission interface to control the light-emitting module. When the mainboard determines that the light board does not support any type of the transmission interfaces indicated by the interface testing signal, the mainboard instructs the lighting control module to control the light-emitting module.