An airtight penetration structure for heat dissipation device includes a first plate member, a second plate member, and a plurality of hollow shaft members. The first and the second plate member are closed to each other to together define a closed chamber between them. The hollow shaft members are respectively provided at two free ends with a first and a second flange. The hollow shaft members are correspondingly extended through fastening holes provided on the first and the second plate member with the first and the second flanges attached to and flush with outer surfaces of the first and the second plate member to seal around the fastening holes, so that the closed chamber between the first and the second plate member is in an airtight state.