Invention Grant
- Patent Title: Maltooligosyl trehalose trehalohydrolase (MTHase) mutant and application thereof
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Application No.: US15991370Application Date: 2018-05-29
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Publication No.: US10465177B2Publication Date: 2019-11-05
- Inventor: Jing Wu , Lingqia Su , Zhendong Wang
- Applicant: Jiangnan University
- Applicant Address: CN wuxi, Jiangsu
- Assignee: Jiangnan University
- Current Assignee: Jiangnan University
- Current Assignee Address: CN wuxi, Jiangsu
- Agency: IPro, PLLC
- Agent Na Xu; Qian Gu
- Priority: CN201810145937 20180212
- Main IPC: C12N9/24
- IPC: C12N9/24 ; C12P19/14 ; C12P19/12
Abstract:
The present invention discloses a maltooligosyl trehalose trehalohydrolase (MTHase) mutant and application thereof, belonging to the technical fields of gene engineering and enzyme engineering. The present invention provides a series of MTHase mutants to prepare trehalose, having a better effect. Further, the MTHase mutant is expressed in Escherichia coli BL21 (DE3) and the enzyme is optimized by fermentation, which can significantly increase the yield of enzymes.
Public/Granted literature
- US20190249158A1 Maltooligosyl Trehalose Trehalohydrolase (MTHase) Mutant and Application Thereof Public/Granted day:2019-08-15
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