Invention Grant
- Patent Title: Genetically encoded probe for quantification of lactate and methods for quantifying metabolic rates and lactate transport
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Application No.: US14394018Application Date: 2012-04-13
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Publication No.: US10466233B2Publication Date: 2019-11-05
- Inventor: Luis Felipe Barros Olmedo , Alejandro San Martin , Sebastian Ceballo Charpentier , Wolf B. Frommer
- Applicant: Luis Felipe Barros Olmedo , Alejandro San Martin , Sebastian Ceballo Charpentier , Wolf B. Frommer
- Applicant Address: CL Valdivia US DC Washington
- Assignee: CENTRO DE ESTUDIOS CIENTIFICOS DE VALDIVIA,CARNEGIE INSTITUTION OF WASHINGTON
- Current Assignee: CENTRO DE ESTUDIOS CIENTIFICOS DE VALDIVIA,CARNEGIE INSTITUTION OF WASHINGTON
- Current Assignee Address: CL Valdivia US DC Washington
- Agency: Merchant & Gould P.C.
- International Application: PCT/US2012/033639 WO 20120413
- International Announcement: WO2013/154587 WO 20131017
- Main IPC: G01N33/542
- IPC: G01N33/542 ; G01N33/50 ; C12N15/85 ; G01N33/58
Abstract:
A nanosensor for detecting and quantifying lactate in different types of samples, such as tissues, intra-cellular and subcellular compartments, with high spatial and temporal resolution is disclosed. Methods comprising use of the nanosensor for quantifying the activity of lactate transporters, rates of cellular lactate production and cellular lactate consumption, and rate of mitochondrial pyruvate consumption are also disclosed. Methods for quantifying the transformation in energy metabolism that characterizes cancer cells with single-cell resolution and for detecting interference of candidate drugs with mitochondrial energetics are additionally disclosed.
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