This disclosure provides an organic light-emitting diode display substrate, and a display apparatus and a production method thereof. The organic light-emitting diode display substrate of this disclosure comprises a blue pixel and a first color pixel, wherein first color light-emitting unit is provided in the first color pixel, and the first color light-emitting unit sequentially comprises a first electrode, a first functional layer, a first color light-emitting layer, a first carrier generation layer, a first blue light-emitting layer, a second carrier generation layer, a second blue light-emitting layer, a second functional layer, and a second electrode in a first direction, and comprises a first color filter; and a blue light-emitting unit is provided in the blue pixel, and the blue light-emitting unit sequentially comprises the first electrode, the first functional layer, the first carrier generation layer, the first blue light-emitting layer, the second carrier generation layer, the second blue light-emitting layer, the second functional layer, and the second electrode in the first direction.