A power-up signal generation circuit including a pre-power-up signal generation block operates by using a first power supply voltage, and generates a pre-power-up signal when the first power supply voltage becomes higher than a first level, and a second power supply voltage becomes higher than a second level; a level shifting block suitable for pull-down driving a first node when the pre-power-up signal is not in an activated state, and pull-up driving the first node with the second power supply voltage when the pre-power-up signal is in the activated state; a driving block suitable for pull-down driving the first node when the second power supply voltage is lower than the second level; and a power-up signal driving block operates by using the second power supply voltage, and generates a power-up signal through a second node by driving the second node based on a voltage level of the first node.