A pixel compensation method, pixel compensation apparatus and display device are provided. In the blanking section of (2n−1)th display frame, the detection line corresponding to the first sub-pixel column of the same color sub-pixels in the nth row is charged with an additional detection voltage, such that the detected voltage on this detection line is the sum of the detection voltage and a coupling voltage. The detection line corresponding to the second sub-pixel column of the same color sub-pixels is not charged with the additional detection voltage, such that the detected voltage on this detection line is the coupling voltage. The detection voltage corresponding to the first sub-pixel column is obtained according to the voltage on the detection line corresponding to each of the same color sub-pixels. Similarly, in the blanking section of (2n)th display frame, the detection voltage corresponding to the second sub-pixel column may be obtained.