Invention Grant
- Patent Title: Simple detection method for RNA modification, and method for detecting type-II diabetes using said detection method
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Application No.: US14772969Application Date: 2014-03-06
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Publication No.: US10526654B2Publication Date: 2020-01-07
- Inventor: Kazuhito Tomizawa , Fanyan Wei , Takeo Suzuki , Tsutomu Suzuki
- Applicant: National University Corporation Kumamoto University
- Applicant Address: JP Kumamoto
- Assignee: NATIONAL UNIVERSITY CORPORATION KUMAMOTO UNIVERSITY
- Current Assignee: NATIONAL UNIVERSITY CORPORATION KUMAMOTO UNIVERSITY
- Current Assignee Address: JP Kumamoto
- Agency: Hunton Andrews Kurth LLP
- Priority: JP2013-047278 20130308; JP2013-150133 20130719
- International Application: PCT/JP2014/055758 WO 20140306
- International Announcement: WO2014/136870 WO 20140912
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12Q1/6883 ; G16B20/00
Abstract:
An object of the present invention is to provide a method for detecting a modification present in RNA using a small amount of RNA sample. Another object of the present invention is to provide a method for detecting a modification present in tRNA, for example, thiomethylation. Still another object of the present invention is to provide a method for detecting the thiomethylation of tRNA, thereby diagnosing human type 2 diabetes or the risk thereof. The present invention is characterized in that, with respect to a modification present in RNA in an RNA sample, cDNA is produced by reverse transcription of RNA using a first primer, and the resulting amount of cDNA is compared with the amount of cDNA produced by reverse transcription of RNA using a second primer, thereby detecting a modification (e.g., thiomethylation) present in RNA.
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