Invention Grant
- Patent Title: Recombinant nucleoside-specific ribonuclease and method of producing and using same
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Application No.: US15568260Application Date: 2016-04-25
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Publication No.: US10533163B2Publication Date: 2020-01-14
- Inventor: Sarah Venus , Balasubrahmanyam Addepalli , Nicholas Paul Lesner , Patrick Alan Limbach
- Applicant: University Of Cincinnati
- Applicant Address: US OH Cincinnati
- Assignee: University of Cincinnati
- Current Assignee: University of Cincinnati
- Current Assignee Address: US OH Cincinnati
- Agency: Wood, Herron & Evans, LLP
- International Application: PCT/US2016/029151 WO 20160425
- International Announcement: WO2016/172678 WO 20161027
- Main IPC: C12N9/22
- IPC: C12N9/22 ; C12Q1/6869 ; C12Q1/6806
Abstract:
A recombinant ribonuclease is disclosed. The recombinant ribonuclease is produced by introducing a recombinant DNA sequence into a host; activating expression of the recombinant DNA sequence within the host to produce the recombinant ribonuclease; and isolating the recombinant ribonuclease from the host. Additionally, a method of analyzing an RNA sequence includes digesting the RNA with a first recombinant ribonuclease to give digestion products comprising nucleotides of the RNA sequence; and analyzing the digestion products using an analytical method to provide the identity of at least some of the nucleotides. The recombinant ribonuclease includes at least one of a uridine-specific recombinant RNase MC1 and a cytidine-specific recombinant RNase Cusativin.
Public/Granted literature
- US20180320154A1 RECOMBINANT NUCLEOSIDE-SPECIFIC RIBONUCLEASE AND METHOD OF PRODUCING AND USING SAME Public/Granted day:2018-11-08
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