It refers to an artificial insemination device which comprises a tube and a tip engageable at one end of the tube, the tip being formed by a hollow tubular body with a semi-spherical upper end provided with at least three orifices; the tip internally comprises at least a first frustoconical portion tapering towards the upper end for insertion of a first vane containing biological material to be inseminated, followed by a first annular portion for coupling this vane, a second frustoconical portion tapering toward the upper end, arranged next to the first annular portion for insertion of a second vane containing biological material having a smaller diameter than the first vane, a second annular portion, arranged after the second additional frustoconical portion, for coupling with the second vane smaller than the first one, and a niche in the region of the upper semi-spherical end where the at least three orifices are arranged.