Provided are a method, target and application for NO accumulation to decrease invasiveness of Pseudomonas aeruginosa; achieving NO accumulation by means of external NO donors or disruption of internal NO metabolism in an environment where Pseudomonas aeruginosa is growing or being cultured, in order to decrease production by Pseudomonas aeruginosa of the invasion factor pyocyanin. Utilizing even micro-quantities of NO can significantly inhibit Pseudomonas aeruginosa PCN (PCN synthesis down 82% with 60 μM SNP treatment); by inhibiting NO reductase, an enzyme related to NO metabolism, PCN synthesis can be significantly inhibited (the PAO1-Δnor strain has 84% PCN reduction). Using NO donors or inhibiting enzymes involved in Pseudomonas aeruginosa NO metabolism as a means of combating bacterial infectious disease does not affect the body's normal microflora like traditional antibiotics.