An assembly structure is provided. A first material layer is disposed on a substrate. A hydrophobic layer is chemically attached to the first material layer. A patterned second material layer is disposed on the substrate, without the hydrophobic or slightly chemically attached with hydrophobic molecules and surrounded by the first material layer. A close-loop sealant wall is directly disposed on the patterned second material layer. A cover layer is directly disposed on the close-loop sealant wall to entirely cover the close-loop sealant wall.