Invention Grant
- Patent Title: Method for sorting cardiomyocytes
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Application No.: US15127134Application Date: 2015-03-20
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Publication No.: US10538740B2Publication Date: 2020-01-21
- Inventor: Yoshinori Yoshida , Hirohide Saito , Kenji Miki , Kei Endo , Seiya Takahashi
- Applicant: KYOTO UNIVERSITY
- Applicant Address: JP Kyoto
- Assignee: Kyoto University
- Current Assignee: Kyoto University
- Current Assignee Address: JP Kyoto
- Agency: Myers Bigel, P.A.
- Priority: JP2014-058926 20140320
- International Application: PCT/JP2015/058466 WO 20150320
- International Announcement: WO2015/141827 WO 20150924
- Main IPC: C12N5/077
- IPC: C12N5/077 ; C12N5/00 ; C12N5/0735 ; A61K48/00

Abstract:
An object of the present invention is to provide a novel method for sorting cardiomyocytes. Another object of the present invention is to provide a method for producing high-purity cardiomyocytes and a kit used therefor. The present invention provides a method for sorting cardiomyocytes, comprising a step of introducing miRNA-responsive mRNA into a cell group, wherein the miRNA-responsive mRNA consists of a sequence comprising the following (i) and (ii): (i) a nucleic acid specifically recognized by miRNA specifically expressed in cardiomyocytes, and (ii) a nucleic acid corresponding to the coding region of a gene, wherein translation of (ii) the nucleic acid corresponding to the coding region of a gene into protein is regulated by the nucleic acid sequence in (i) above, thereby achieving the aforementioned objects.
Public/Granted literature
- US20170369846A1 METHOD FOR SORTING CARDIOMYOCYTES Public/Granted day:2017-12-28
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