A substrate for sample analysis on which transfer of a liquid is to occur with rotational motion includes: a substrate having a rotation axis; a first chamber being located in the substrate and having a first space for retaining a liquid and magnetic particles; a second chamber being located in the substrate and having a second space for retaining the liquid to be discharged from the first chamber; a channel being located in the substrate and having a path connecting the first chamber and the second chamber; and a magnet being located in the substrate and near the first space in the substrate for capturing the magnetic particles in the first chamber.