A probe assembly and a detecting device including the same, the probe assembly is configured to detect the display panel and includes a first probe region corresponding to leads of the display panel, wherein a location in the first probe region corresponding to a first lead among the leads connected in the display panel is provided with a probe, and a location in the first probe region corresponding to a lead adjacent to the first lead is not provided with a probe; wherein the first lead is any one of the leads which are connected. The detecting device comprises the probe assembly. It solves the problem that the currently known probe assembly may result in short-circuit between adjacent probes when used for display panel detection, avoids the condition where a detective display panel cannot be detected, improves the detection rate, ensures the image quality of the display panel, and hence avoids a waste of production material and reduces the production cost.