Methods and apparatus to manage configuration of policy-based hypervisors are disclosed. An example method includes generating, by executing an instruction with a processor, a specification for a target configuration of a host based on a policy-based profile, the policy-based profile describing a source configuration of a source host at a virtualization software level; applying, by executing an instruction with the processor, the specification to the target configuration; and in response to an update to the policy-based profile, updating, by executing an instruction with the processor, the target configuration to place the host in compliance with the updated policy-based profile.