This invention relates to a patterned substrate for light emitting diode. The patterned substrate includes a surface having a plurality of protrusive structures, wherein each of the protrusive structures includes an upper portion and a lower portion. The surface of the lower portion is a conical surface, and has at least one first zone and at least one second zone. The first zone and the second zone are alternately arranged, wherein the first zone has one or more first projections.