Invention Grant
- Patent Title: Selection marker for cell transfection and protein production
-
Application No.: US15520840Application Date: 2015-10-22
-
Publication No.: US10557125B2Publication Date: 2020-02-11
- Inventor: Bruno Dumas , Nabil Lounis
- Applicant: Sanofi
- Applicant Address: FR Paris
- Assignee: SANOFI
- Current Assignee: SANOFI
- Current Assignee Address: FR Paris
- Agency: McDonnell Boehnen Hulbert & Berghoff LLP
- Priority: EP14306689 20141023
- International Application: PCT/EP2015/074550 WO 20151022
- International Announcement: WO2016/062837 WO 20160428
- Main IPC: C12N9/02
- IPC: C12N9/02 ; C12N15/85 ; C12N15/65 ; C12P21/00 ; C12N15/09 ; C12N1/20 ; C12N15/00 ; C12P21/06 ; C12P21/04 ; C07H21/04 ; A61K38/00
Abstract:
The present invention is within the field of industrial protein production. The invention provides a novel expression system using dihydroorotate dehydrogenase (DHODH) as a selection marker in combination with leflunomide or a metabolite thereof, notably for use in mammalian cell lines. Expression vectors encoding DHODH, cell lines comprising said vectors and methods of producing recombinant proteins are also provided.
Public/Granted literature
- US20170335292A1 Novel Selection Marker for Cell Transfection and Protein Production Public/Granted day:2017-11-23
Information query
