A method of determining beryllium or a beryllium compound thereof in a sample is disclosed by measuring fluorescence. This method discloses use of highly alkaline fluorescent indicating dye solutions with pH greater than 12.9. In a preferred embodiment the fluorescent indicating dye solutions do not contain a pH buffer. Further, the method also discloses measuring fluorescence under highly alkaline conditions, where the pH after mixing the highly alkaline fluorescent indicating dye solutions with the sample solution containing beryllium is at least 11, preferably 12. The use of highly alkaline solution provides superior detection limits for beryllium by using dilution ratios of 4× and lower.