Invention Grant
- Patent Title: Method for inducing differentiation of pluripotent stem cells into germ cells
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Application No.: US15740454Application Date: 2016-06-29
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Publication No.: US10563171B2Publication Date: 2020-02-18
- Inventor: Mitinori Saitou , Kotaro Sasaki , Shihori Yokobayashi
- Applicant: Kyoto University
- Applicant Address: JP Kyoto
- Assignee: Kyoto University
- Current Assignee: Kyoto University
- Current Assignee Address: JP Kyoto
- Agency: Leydig, Voit & Mayer, Ltd.
- Priority: JP2015-130501 20150629
- International Application: PCT/JP2016/069360 WO 20160629
- International Announcement: WO2017/002888 WO 20170105
- Main IPC: C12N5/0735
- IPC: C12N5/0735 ; G01N33/569

Abstract:
The invention provides a method for inducing human primordial germ cell-like (PGC-like) cells from human pluripotent stem cells, with high efficiency and high reproducibility, and a cell surface marker for identifying human PGC-like cells. In particular, the invention provides a method for producing a human PGC-like cell from a human pluripotent stem cell, includes a step of producing a mesoderm-like cell by culturing a human pluripotent stem cell in a culture medium comprising activin A and a GSK3β inhibitor, and a step of culturing the mesoderm-like cell in a culture medium containing BMP. The invention also provides a method for producing an isolated human PGC-like cell, which includes the aforementioned two steps and the additional step of selecting a cell positive to at least one cell surface marker selected from the group consisting of PECAM (CD31), INTEGRINα6 (CD49f), INTEGRINβ3 (CD61), KIT (CD117), EpCAM, PODOPLANIN and TRA1-81.
Public/Granted literature
- US20180187147A1 METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO GERM CELLS Public/Granted day:2018-07-05
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