A system and methods for RF (Radio Frequency) penetration imaging of one or more objects in a medium, the system including a generation and reception subsystem configured to generate and receive a plurality of RF signals, an RF antenna array including a plurality of antennas, the antennas being configured to transmit the RF signals towards the medium and receive a plurality of RF signals reflected from the medium, a data acquisition subsystem configured to receive and store the reflected RF signals, and a processor configured to estimate the distance between the surface of the target medium and the antenna array, the delay between the transmitted signals and the plurality of signals reflected from the object using a dedicated frequency sub-band of the received signals, the location of the antennas at each transmitting time, and determine whether there is an object within the medium.