Invention Grant
- Patent Title: Artificial antigen produced using partial sequence of enolase protein originated from plasmodium falciparum, and method for producing same
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Application No.: US15531122Application Date: 2015-11-27
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Publication No.: US10583184B2Publication Date: 2020-03-10
- Inventor: Hiroyuki Oku , Shigeyuki Kano , Kazuhiko Yano
- Applicant: Shigeyuki Kano
- Applicant Address: JP Tokyo
- Assignee: Shigeyuki Kano
- Current Assignee: Shigeyuki Kano
- Current Assignee Address: JP Tokyo
- Agency: Leydig, Voit & Mayer, Ltd.
- Priority: JP2014-241420 20141128
- International Application: PCT/JP2015/083437 WO 20151127
- International Announcement: WO2016/084944 WO 20160602
- Main IPC: A61K39/00
- IPC: A61K39/00 ; A61K39/02 ; A61K39/015 ; C12N9/88 ; G01N33/53 ; A61K38/00 ; C07K14/445 ; A61K47/64 ; A61K9/16 ; G01N33/543 ; G01N33/569 ; G01N33/68

Abstract:
The invention provides a bioactive peptide including a partial amino acid sequence of Plasmodium falciparum enolase, and having a molecular structure compatible with a specification setting for a GMP-compliant production process. The peptide has a structure in which two peptides, each having an amino acid sequence of A01-Ala-Ser-Glu-Phe-Tyr-Asn-Ser-Glu-Asn-Lys-Thr-Tyr-Asp-Leu-Asp-Phe-Lys-Thr-Pro-Asn-Asn-Asp-A02 (SEQ ID NO: 1) or A03-Ala-Ser-Glu-Phe-Tyr-Asn-Ser-Glu-Asn-Lys-Thr-Tyr-Asp-Leu-Asp-Phe-Lys-Thr-Pro-Asn-Asn-Asp-Lys-Ser-Leu-Val-Lys-Thr-A04 (SEQ ID NO: 2) are linked by amide bonds between the respective carboxy termini of the two peptides and two amino groups of Lys in a linker peptide represented by Lys-A05-Cys-A06 and arranged in the form of a two-forked branch, wherein each of A01 to A06 represents an amino acid residue in a number of an arbitrary number including 0. The peptide preferably has a dimerized structure in which two of the above described peptides are linked by an S—S bond between the Cys residues in the linker peptide sequences included in the respective two peptides.
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