An automatic travel vehicle includes a body, a drive unit to move the body on a travel surface along a travel guide line, a detection unit to detect a mark attached to one adjacent portion of the travel guide line for one spot set along the travel guide line, and a control unit to control an operation of the automatic travel vehicle based on the detected mark. The control unit interprets the mark attached to the one adjacent portion of the travel guide line at a first side of the body when the automatic travel vehicle travels along the travel guide line in a first travelling direction, and interprets the mark attached to the one adjacent portion of the travel guide line at a second side of the body when the automatic travel vehicle travels along the travel guide line in a second travelling direction.