Invention Grant
- Patent Title: Trichoderma fungus having mutant-type BXL1 gene and method of producing xylooligosaccharide and glucose by using same
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Application No.: US16089271Application Date: 2017-03-30
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Publication No.: US10590497B2Publication Date: 2020-03-17
- Inventor: Koji Kobayashi , Shingo Hiramatsu , Katsushige Yamada
- Applicant: Toray Industries, Inc.
- Applicant Address: JP Tokyo
- Assignee: Toray Industries, Inc.
- Current Assignee: Toray Industries, Inc.
- Current Assignee Address: JP Tokyo
- Agency: DLA Piper LLP (US)
- Priority: JP2016-070690 20160331
- International Application: PCT/JP2017/013379 WO 20170330
- International Announcement: WO2017/170918 WO 20171005
- Main IPC: C13K1/02
- IPC: C13K1/02 ; C12N1/14 ; C12N15/01 ; C13K13/00 ; C12N15/09 ; C12N9/24 ; C12P19/04 ; C12P19/14 ; C12R1/885

Abstract:
A novel fungus belongs to the genus Trichoderma whose cellulase can be used to hydrolyze cellulose-based biomass without degradation of xylan contained in the biomass into xylose, and a method produces glucose and xylo-oligosaccharides from a cellulose containing biomass using it. The fungus belonging to the genus Trichoderma includes N- and C-terminal domains of the β-xylosidase 1 (BXL1) gene, but lacks the Fn3-like domain of the gene due to its disruption. The use of this fungus belonging to the genus Trichoderma results in deletion of β-xylosidase activity and increase in β-glucosidase activity. Thus, in hydrolysis of cellulose contained in biomass, xylan contained in the biomass is not degraded into xylose, which enables efficient production of glucose and xylo-oligosaccharides.
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