A flow control assembly for an agricultural metering system includes a first gate control rod configured to couple to a first gate of a seed meter and a second gate control rod configured to couple to a second gate of the seed meter. The flow control assembly also includes a locking assembly configured to block translation of the first gate control rod at least while the second gate control rod is in a positioned that corresponds to an open position of the second gate and to block translation of the second gate control rod at least while the first gate control rod is in a position that corresponds to an open position of the first gate.