A low vibration and noise reduction exercise mat unit has a buffer plate and multiple protruding portions. The buffer plate has a board, multiple male buckles, and multiple female buckles. The board is quadrilateral and has a bottom, a recess, and a flange. The recess is formed on a bottom surface of the board. The flange is formed around the recess. The multiple protruding portions are disposed at spaced intervals in the recess of the board. The multiple protruding portions protrude downwardly from an inner top surface of the recess of the board. The buffer plate and the multiple protruding portions are made of natural rubber, will not affect the human body, and are stable in quality. The male buckles and female buckles can enhance the connecting strength between the mat units.