Invention Grant
- Patent Title: Method of determining a nucleotide sequence of a nucleic acid segment from a region of interest in a biological sample
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Application No.: US15003450Application Date: 2016-01-21
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Publication No.: US10597697B2Publication Date: 2020-03-24
- Inventor: John Richard Nelson , Qingyan Au , Wei Gao , Ryan Charles Heller , Nicholas Hoe , Nam Tran , Nichole Lea Wood
- Applicant: GE Healthcare Bio-Sciences Corp.
- Applicant Address: US MA Marlborough
- Assignee: GE Healthcare Bio-Sciences Corp.
- Current Assignee: GE Healthcare Bio-Sciences Corp.
- Current Assignee Address: US MA Marlborough
- Agency: Eversheds Sutherland (US) LLP
- Main IPC: G01N33/53
- IPC: G01N33/53 ; C12Q1/6806 ; G01N33/58
Abstract:
The invention discloses a method for determining a nucleotide sequence of a nucleic acid segment present in a biological sample, comprising the steps of: a) generating a fluorescent image of the sample by a protocol comprising immunofluorescence detection of at least five different target proteins in the sample; b) selecting a region of interest of the sample by comparing the image to a predetermined criterion; c) removing a subsample from the region of interest, and; d) determining a nucleotide sequence of a nucleic acid segment present in the subsample.
Public/Granted literature
- US20160230219A1 METHOD FOR NUCLEIC ACID ANALYSIS FROM A REGION OF INTEREST IN A BIOLOGICAL SAMPLE Public/Granted day:2016-08-11
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