Invention Grant
- Patent Title: Copy number preserving RNA analysis method
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Application No.: US15127672Application Date: 2015-03-20
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Publication No.: US10597699B2Publication Date: 2020-03-24
- Inventor: Pamela Moll
- Applicant: LEXOGEN GMBH
- Applicant Address: AT Vienna
- Assignee: LEXOGEN GMBH
- Current Assignee: LEXOGEN GMBH
- Current Assignee Address: AT Vienna
- Agency: Wolf, Greenfield & Sacks, P.C.
- Priority: EP14161135 20140321
- International Application: PCT/EP2015/055961 WO 20150320
- International Announcement: WO2015/140307 WO 20150924
- Main IPC: C12Q1/6806
- IPC: C12Q1/6806 ; C12Q1/6851 ; C12Q1/6853 ; C12Q1/686 ; C12N15/10 ; C40B20/04

Abstract:
The present invention provides a method for generating an amplified nucleic acid portion of a template RNA molecule, comprising after having obtained a template RNA, annealing a first oligonucleotide primer at a preselected 3′ terminal nucleic acid region of the template RNA, elongating the first oligonucleotide primer in a template specific manner thereby obtaining a first elongated strand, removing the RNA template, annealing one or more further oligonucleotide primers to the first elongated strand, elongating the one or more further oligonucleotide primers in a template specific manner without strand displacement of polynucleotides annealed to the first elongated strand or using a polymerase that destroys a displaced strand, thereby generating further elongation products, isolating and/or amplifying an elongation product of said further elongation product comprising a nucleic acid portion that is elongated complementary to the first oligonucleotide primer; as well as kits for performing the method.
Public/Granted literature
- US20180171384A1 COPY NUMBER PRESERVING RNA ANALYSIS METHOD Public/Granted day:2018-06-21
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