Disclosed are systems and method for controlling access to objects of an operating system using Access Control Lists (ACLs). An exemplary method comprises: generating, by a processor, one or more ACLs for objects of the operating system based on at least one access rule specifying the access mode to the object of the operating system to one or more users based on the one or more categories to which the objects belongs; intercepting a request from a user to access an object of the operating system; determining, by the processor, one or more ACLs associated with the requested object; and applying, by the processor, the determined one or more ACLs to decide whether to allow or deny access of the user to the requested object, wherein if one of the applied ACLs denies access to the object, the access will be blocked, otherwise the access will be allowed.