Provided is a single-channel chemiluminescent micro-fluidic chip, including a chip body with a quantification-reaction cavity and a waste liquid cavity. The quantification-reaction cavity is composed of a quantification-reaction pool on a lower portion and a reaction pool cover plate, the quantification-reaction pool is equally divided into three cavities by two partition plates, a labeled antibody is placed in the middle cavity, coated antibodies are placed in the others, a surface, facing the quantification-reaction pool, of the reaction pool cover plate is equally divided into two parts. In result, the coated antibodies and the labeled antibodies are physically separated to effectively avoid nonspecific binding. Due to a special structure of the reaction tank, wavy fluid flow is generated to fully mix the coated antibodies and the labeled antibodies, which improves testing efficiency and sensitivity.