A light-emitting control signal generation circuit, a driving method thereof, and a display device are provided. The light-emitting control signal generation circuit includes a first control unit, a second control unit, and an output unit. The first control unit controls a potential-level on a first node under control of an effective pulse signal, a first clock signal, a second clock signal, and a first voltage signal. The second control unit controls a potential-level on a second node under control of the effective pulse signal, the first clock signal, the second clock signal, the first voltage signal, a second voltage signal, and the potential-level on the first node. The output unit transmits the first voltage signal to an signal output terminal under control of the potential-level on the first node, and transmits the second voltage signal to the signal output terminal under control of the potential-level on the second node, respectively.