A tablet computer positioning assembly for stabilizing and positioning a tablet computer includes a plate that is configured to position on a substantially horizontal surface. A first frame is coupled to and extends perpendicularly from the plate proximate to a first side of the plate. A second frame is pivotally coupled to the first frame and extends substantially perpendicularly from the first frame over the plate. The second frame is selectively fixedly couplable to the first frame so that the second frame is selectively angularly positionable relative to the plate. A plurality of couplers that is coupled to the second frame is configured to couple to a rim of a tablet computer to couple the tablet computer to the second frame so that the second frame is configured to selectively position the tablet computer relative to the plate.