A specimen retrieval device includes a deployment assembly and a specimen retrieval bag supported by the deployment assembly. The deployment assembly includes an insertion tube, a shaft slidably receivable through the insertion, a handle disposed on a proximal end portion of the shaft, and a support assembly disposed on a distal end portion of the shaft. The specimen retrieval bag includes a pouch portion supported by the support assembly, a tail portion extending from the pouch portion and secured to the handle, and a closure cord extending through the pouch portion for cinching the pouch portion.