A driving method for a display device, timing controller and display device. The driving method includes: acquiring a target update frequency of image display of a display panel; determining a target frame rate corresponding to the target update frequency according to a prestored corresponding relationship between update frequencies and frame rates; adjusting a blanking duration of a data signal output by a source driving circuit according to the target frame rate; and outputting a first control signal with a frequency being the target frame rate to a gate driving circuit, to cause the gate driving circuit to scan pixel units of the display panel according to the target frame rate.