An AgNW transparent conductive film and manufacturing method, and a touch panel thereof are provided. The AgNW transparent conductive film includes a transparent substrate, and a compound layer positioned on the transparent substrate. The compound layer comprises AgNWs and active materials, partial the active material exposed on surface of the compound layer which are far away the transparent substrate, end of molecular chain of the active material has terminal group could react with acidic group. This disclosure enhances tolerance of photoresist material and chemical liquid which are usually used for wet chemical etching process while produces touch panel. The manufacturing method comprises coating an active function layer doping with active materials on the transparent substrate, and then forming the AgNW on the active function layer, obtaining the compound layer.