A touch panel, a touch detection method thereof, and a display device are provided. The touch panel includes an electrowetting substrate and a touch substrate which are disposed opposite to each other and a conductive fluid drop disposed between the electrowetting substrate and the touch substrate. The electrowetting substrate includes a first base substrate, a control electrode, and a first insulating layer; the conductive fluid drop is disposed at a side of the first insulating layer away from the control electrode, and corresponds to the control electrode; the touch substrate includes a second base substrate and a touch electrode unit disposed at a side of the second base substrate close to the electrowetting substrate; the touch electrode unit and the conductive fluid drop are disposed opposite to each other.