A non-contact visual detection method for Mark positioning of mobile phone touch screen. The method divides the touch screen detection part of the mobile phone into two areas A and B. The black-and-white industrial camera takes phase detection, and the B-zone Mark and Circle are phase-detected by the second black-and-white industrial camera. After taking the picture, the image acquisition module collects the image and transmits the image information to the upper computer module. The upper computer module processes the data, and the image processing algorithm detects the deviation distance between the Mark center point and the Circle center, displays the detection result and gives the completion signal, after which The motion control module returns to the initial position and waits for the next workpiece detection. The detection method can realize fast, accurate and automatic detection of the mobile phone touch screen Mark positioning.